Anti-FHL1 Antibody, clone 3H3

Also for FHL1 (NM_001449)

OriGene Data

Immunogen	Full length human recombinant protein of human FHL1 (NP_001440) produced in HEK293T cell.
Clone Name	clone 3H3	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:2000, IF 1:100, FLOW 1:100
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens four and a half LIM domains 1 (FHL1), transcript variant 2
Alternative Name	FHL-1; FHL1A; FHL1B; FLH1A; KYOT; SLIM; SLIM-1; SLIM1; SLIMMER; XMPMA
Database Link	NP_001440
Function	This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.
Related Pathway	Delta-Notch Signaling Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (RC203478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.

IF Image

Anti-FHL1 mouse monoclonal antibody (TA501375) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1(RC203478).

FC Image

HEK293T cells transfected with either RC203478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FHL1 antibody(TA501375), and then analyzed by flow cytometry.

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Anti-FHL1 TrueMAB Antibody clone 3H3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data