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Anti-FH TRUEMAB Antibody Clone OTI9G4
TrueMAB Antibodies - Made against Authentic Protein Antigens
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|Full length human recombinant protein of human FH (NP_000134) produced in HEK293T cell.|
|Human , Dog , Rat
|WB, IHC, IF, FC
||WB 1:1000, IHC 1:50, IF 1:100, FLOW 1:100,
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens fumarate hydratase (FH)|
|FMRD; HLRCC; LRCC; MCL; MCUL1|
Entrez Gene 2271 Human
Entrez Gene 24368 Rat
Entrez Gene 480092 Dog
|The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq].|
|Druggable Genome Citrate cycle (TCA cycle)Metabolic pathwaysPathways in cancerRenal cell carcinoma|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FH (RC200614, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FH.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-FH monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500682)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500682)
Anti-FH mouse monoclonal antibody (TA500682) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FH(RC200614).
HEK293T cells transfected with either RC200614 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FH antibody(TA500682), and then analyzed by flow cytometry.