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Home Antibody All anti-FH antibodies

Anti-FH TRUEMAB Antibody clone 9G4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500682
  • FH mouse monoclonal antibody, clone 9G4
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400053) , 20ug Explanation
100ul 379 In Stock
CF500682
  • Carrier-free (BSA/glycerol-free) FH mouse monoclonal antibody, clone 9G4
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IHC(2)
IF(1)
FC(1)
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Also for FH (NM_000143)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human FH (NP_000134) produced in HEK293T cell.
Clone Nameclone 9G4 IsotypeIgG1
Species ReactivityHuman , Dog , Rat Concentration0.7 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000, IHC 1:50, IF 1:100, FLOW 1:100,
Predicted MW Explanation 50.2 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens fumarate hydratase (FH)
Alternative NameHLRCC; LRCC; MCL; MCUL1
Database LinkNP_000134
Entrez Gene 2271 Human
Entrez Gene 24368 Rat
Entrez Gene 480092 Dog
FunctionThe protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq].
Related PathwayDruggable Genome Citrate cycle (TCA cycle)Metabolic pathwaysPathways in cancerRenal cell carcinoma

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FH (RC200614, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FH.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-FH monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-FH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500682)
IHC Image
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-FH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500682)
IF Image
Anti-FH mouse monoclonal antibody (TA500682) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FH(RC200614).
FC Image
HEK293T cells transfected with either RC200614 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FH antibody(TA500682), and then analyzed by flow cytometry.

 

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