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Home Antibody All anti-FGFR2 antibodies

Anti-FGFR2 TRUEMAB Antibody Clone OTI3F8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502799 FGFR2 mouse monoclonal antibody, clone OTI3F8 (formerly 3F8) 100ul
30ul
$379 In Stock
LC400050 FGFR2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502799 Carrier-free (BSA/glycerol-free) FGFR2 mouse monoclonal antibody, clone OTI3F8 (formerly 3F8) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(3)
IHC(6)
IF(1)
FC(2)
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Also for FGFR2 (NM_000141)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human FGFR2(NP_000132) produced in HEK293T cell.
Clone NameClone OTI3F8 IsotypeIgG2a
Species ReactivityHuman Concentration0.93 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens fibroblast growth factor receptor 2 (FGFR2), transcript variant 1
Alternative NameBBDS; BEK; BFR-1; CD332; CEK3; CFD1; ECT1; JWS; K-SAM; KGFR; TK14; TK25
Database LinkNP_000132
Entrez Gene 2263 Human
FunctionThe protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq].
Related PathwayProtein KinaseSecreted ProteinTransmembraneDruggable Genome MAPK signaling pathwayEndocytosisRegulation of actin cytoskeletonPathways in cancerProstate cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR2 (RC217098, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2.
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Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-FGFR2 monoclonal antibody at 1:200 dilution.
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Cross-Reactivity of anti-FGFR2 (TA502799) with FGFR1 and FGFR3,not with FGFR4.HEK293T cells were transfected with the DDK(FLAG)-tagged DNAs of FGFR2 (RC217098),FGFR1(RC202080),FGFR3(RC215533),FGFR4(RC204230) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2(TA502799).(1:500) and anti-DDK (1:2000), respectively.
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502799)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502799)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502799)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502799)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502799)
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502799)
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Anti-FGFR2 mouse monoclonal antibody (TA502799) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FGFR2(RC217098).
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Flow cytometric Analysis of Hela cells, using anti-FGFR2 antibody(TA502799),(Red), compared to a nonspecific negative control antibody,(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-FGFR2 antibody(TA502799),(Red), compared to a nonspecific negative control antibody,(Blue).
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