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Anti-FGF21 TRUEMAB Antibody Clone 9E5
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for FGF21 (NM_019113)
|Human recombinant protein fragment corresponding to amino acids 29-209 of human FGF21(NP_061986) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB: 1:2000, IHC: 1:150, FLOW: 1:100, IF: 1:50-1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGF21 (RC204538, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGF21.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-FGF21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502788)
Anti-FGF21 mouse monoclonal antibody (TA502788) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FGF21(RC204538) at 1:100
HEK293T cells transfected with either RC204538 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FGF21 antibody(TA502788), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-FGF21 antibody(TA502788),(Red), compared to a nonspecific negative control antibody,(Blue).