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Anti-BFGF TRUEMAB Antibody clone 3D9
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for BFGF (NM_002006)
|Human recombinant protein fragment corresponding to amino acids 10-155 of human bFGF (NP_001997) produced in E.coli.|
|WB, IHC, IF
||WB 1:1000, IHC 1:50, IF 1:100,
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens fibroblast growth factor 2 (basic) (FGF2)|
|BFGF; FGF-2; FGFB; HBGF-2|
Entrez Gene 2247 Human
|BFGF is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.|
|Secreted ProteinDruggable Genome MAPK signaling pathwayRegulation of actin cytoskeletonPathways in cancerMelanoma|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFGF (RC217426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFGF.
Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-BFGF monoclonal antibody at 1:200 dilution.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-BFGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500012)
Anti-BFGF mouse monoclonal antibody (TA500012) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BFGF(RC217426).