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Anti-FERMT2 TRUEMAB Antibody Clone OTI22E7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500509 anti-FERMT2 mouse monoclonal antibody, clone OTI22E7 (formerly 22E7) 100ul 325
In Stock
LC416377 FERMT2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500509 Carrier-free (BSA/glycerol-free) FERMT2 mouse monoclonal antibody, clone OTI22E7 (formerly 22E7) 100ug $450 3-4 weeks
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Also for FERMT2 (NM_006832)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human FERMT2 (NP_006823) produced in HEK293T cell.
Clone NameClone OTI22E7 IsotypeIgG2a
Species ReactivityHuman Concentration0.88 mg/ml
Guaranteed Application *WB, IF, IP Suggested DilutionsWB: 1:500, IF: 1:100, IP: 4ug/mL
Predicted MW Explanation 77.9 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens fermitin family member 2 (FERMT2), transcript variant 1
Alternative NameKIND2; mig-2; MIG2; PLEKHC1; UNC112; UNC112B
Database LinkNP_006823
Entrez Gene 10979 Human
FunctionParticipates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.

WB Image
Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-FERMT2 monoclonal antibody.(1:200)

IF Image
Anti-FERMT2 mouse monoclonal antibody (TA500509) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FERMT2(RC205727).

IF Image
Immunofluorescent staining of HeLa cells using anti-FERMT2 mouse monoclonal antibody (TA500509).

IP Image
Immunoprecipitation of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.


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