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Anti-FER Antibody EP1842Y
|A synthetic peptide corresponding to residues on the N-terminus of human Fer was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IP, FC
||WB: 1:5000; IP: Use a concentration of 5 ug/ml; FC: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC or IHC-P.
|Homo sapiens fer (fps/fes related) tyrosine kinase (FER)|
|FerT; PPP1R74; TYK3|
|Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. [provided by RefSeq]. |
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Western blot - FER antibody [EP1842Y]; Anti-FER antibody [EP1842Y] at 1/5000 dilution + Jurkat cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 93 kDa.Observed band size : 93 kDa.
Immunoprecipitation - Anti-FER antibody [EP1842Y]; FER was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to FER and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). .The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52479.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: 93kDa; FER
Flow Cytometry-Anti-FER antibody [EP1842Y](ab52479); Overlay histogram showing Jurkat cells stained with ab52479 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.