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Home Antibody All anti-FDFT1 antibodies

Anti-FDFT1 TRUEMAB Antibody Clone OTI1H9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA503450 FDFT1 mouse monoclonal antibody, clone OTI1H9 (formerly 1H9) 100ul $325 In Stock
LC401419 FDFT1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503450 Carrier-free (BSA/glycerol-free) FDFT1 mouse monoclonal antibody, clone OTI1H9 (formerly 1H9) 100ug $450 3-4 weeks
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WB(2)
IHC(4)
IF(1)
FC(1)
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Also for FDFT1 (NM_004462)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human FDFT1(NP_004453) produced in HEK293T cell.
Clone NameClone OTI1H9 IsotypeIgG2b
Species ReactivityHuman Concentration1.1 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 47.9 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens farnesyl-diphosphate farnesyltransferase 1 (FDFT1), transcript variant 3
Alternative NameDGPT; ERG9; SQS; SS
Database LinkNP_004453
Entrez Gene 2222 Human
FunctionThis gene encodes a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene. [provided by RefSeq].
Related PathwayDruggable Genome Steroid biosynthesisMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FDFT1 (RC201392, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FDFT1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FDFT1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FDFT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503450)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FDFT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503450)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FDFT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503450)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FDFT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503450)
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Anti-FDFT1 mouse monoclonal antibody (TA503450) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FDFT1(RC201392).
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HEK293T cells transfected with either RC201392 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FDFT1 antibody(TA503450), and then analyzed by flow cytometry.
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