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Anti-FBXO42 TRUEMAB Antibody Clone 2G7
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for FBXO42 (NM_018994)
|Human recombinant protein fragment corresponding to amino acids 356-717 of human FBXO42 (NP_061867) produced in E.coli.|
|WB, IHC, FC
|| WB 1:500~2000, IHC 1:150, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens F-box protein 42 (FBXO42)|
|Members of the F-box protein family, such as FBXO42, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (SKP1A; MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Dec 2010]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXO42 (RC209600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXO42.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FBXO42 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-FBXO42 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA800210)
HEK293T cells transfected with either RC209600 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FBXO42 antibody(TA800210), and then analyzed by flow cytometry.