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Home Antibody All anti-FBXO21 antibodies

Anti-FBXO21 TRUEMAB Antibody Clone OTI5H4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA504025 FBXO21 mouse monoclonal antibody, clone OTI5H4 (formerly 5H4) 100ul $325 In Stock
CF504025 Carrier-free (BSA/glycerol-free) FBXO21 mouse monoclonal antibody, clone OTI5H4 (formerly 5H4) 100ug $450 3-4 weeks
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WB(2)
IHC(10)
IF(1)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human FBXO21(NP_296373) produced in HEK293T cell.
Clone NameClone OTI5H4 IsotypeIgG1
Species ReactivityHuman , Rat , Monkey , Mouse Concentration0.54 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 72.1 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens F-box protein 21 (FBXO21), transcript variant 1
Alternative NameFBX21
Database LinkNP_296373
Entrez Gene 23014 Human
Entrez Gene 231670 Mouse
Entrez Gene 360818 Rat
Entrez Gene 693647 Monkey
FunctionThis gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXO21 (RC223095, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXO21.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FBXO21 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504025)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504025)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504025)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504025)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504025)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504025)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504025)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504025)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504025)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504025)
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Anti-FBXO21 mouse monoclonal antibody (TA504025) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FBXO21(RC223095).
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HEK293T cells transfected with either RC223095 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FBXO21 antibody(TA504025), and then analyzed by flow cytometry.
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