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Anti-FBXO21 TRUEMAB Antibody Clone OTI2B8
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for FBXO21 (NM_033624)
|Full length human recombinant protein of human FBXO21(NP_269373) produced in HEK293T cell.|
|Human, Dog, Rat, Monkey
|WB, IHC, IF, FC
||WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens F-box protein 21 (FBXO21), transcript variant 1|
Entrez Gene 23014 Human
Entrez Gene 360818 Rat
Entrez Gene 693647 Monkey
Entrez Gene 486290 Dog
|This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXO21 (RC223095, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXO21.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FBXO21 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504016)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504016)
Anti-FBXO21 mouse monoclonal antibody (TA504016) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FBXO21(RC223095).
HEK293T cells transfected with either RC223095 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FBXO21 antibody(TA504016), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-FBXO21 antibody(TA504016),(Red), compared to a nonspecific negative control antibody,(Blue).