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Anti-FABP3 GAT Antibody Polyclonal
Generated by Genomic Immunization
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Also for FABP3 (NM_004102)
|DNA immunization. This antibody is specific for the entire range of the target protein.|
|Human , Dog
|WB, ELISA, IHC, IF
|| WB 1:5000~20000, IHC 1:150, ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens fatty acid binding protein 3, muscle and heart (FABP3)|
|FABP11; H-FABP; M-FABP; MDGI; O-FABP|
Entrez Gene 2170 Human
Entrez Gene 478156 Dog
|The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer.|
| PPAR signaling pathway|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FABP3 (RC202737, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FABP3.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FABP3 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-FABP3 rabbit polyclonal antibody. (TA590642)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-FABP3 rabbit polyclonal antibody. (TA590642)
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-FABP3 rabbit polyclonal antibody. (TA590642)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FABP3 rabbit polyclonal antibody. (TA590642)
Immunohistochemical staining of human skeletal muscle shows cytoplasmic positivity in myocytes.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line A-431 shows positivity in cytoskeleton (intermediate filaments).This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.