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Home Antibody All anti-EphB1 antibodies

Anti-EphB1 Antibody EPR6457


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal antibody against EphB1
100ul 325 In Stock
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Also for EphB1 (NM_004441)
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human EphB1 was used as an immunogen.
Clone NameEPR6457 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IF Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Reference Data

Target NameHomo sapiens EPH receptor B1 (EPHB1)
Alternative NameELK; EPHT2; Hek6; NET
Database LinkNP_004432
FunctionEphrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB1 is a receptor for ephrin-B family members.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Eph receptor B1 antibody [EPR6457]; All lanes : Anti-Eph receptor B1 [EPR6457] antibody at 1/1000 dilution.Lane 1 : MDA-MB-435 cell lysate.Lane 2 : U-87MG cell lysate.Lane 3 : NIH 3T3 cell lysate.Lane 4 : COS1 cell lysate.Lane 5 : Human fetal brain cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 110 kDa.
Assay Image
Other-Anti-Eph receptor B1 antibody [EPR6457](TA310460); Equilibrium disassociation constant (KD)..
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B1 antibody [EPR6457]; Immunofluorescence analysis of rat oligodendrocytes, staining Eph receptor B1 with TA310460. Cells were fixed with formaldehyde, permeabilized with 0.03% Triton X-100 and blocked with 1% serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/300 in serum + 0.01% Triton X-100) for 10 hours at 4°C. An AlexaFluor?555-conjugated donkey anti-rabbit polyclonal IgG (1/300) was used as the secondary antibody.


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