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Home Antibody All anti-EphB1 antibodies

Anti-EphB1 Antibody EPR6457

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310460
  • Rabbit Monoclonal antibody against EphB1
100ul 325 In Stock
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WB(1)
IF(1)
Assay(1)
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Also for EphB1 (NM_004441)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human EphB1 was used as an immunogen.
Clone NameEPR6457 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IF Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Reference Data

Target NameHomo sapiens EPH receptor B1 (EPHB1)
Alternative NameELK; EPHT2; Hek6; NET
Database LinkNP_004432
Entrez Gene 2047 Human
Entrez Gene 270190 Mouse
Entrez Gene 24338 Rat
FunctionEphrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB1 is a receptor for ephrin-B family members.
Related PathwayProtein KinaseTransmembraneDruggable Genome Axon guidance

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Eph receptor B1 antibody [EPR6457]; All lanes : Anti-Eph receptor B1 [EPR6457] antibody at 1/1000 dilution.Lane 1 : MDA-MB-435 cell lysate.Lane 2 : U-87MG cell lysate.Lane 3 : NIH 3T3 cell lysate.Lane 4 : COS1 cell lysate.Lane 5 : Human fetal brain cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 110 kDa.
Assay Image
Other-Anti-Eph receptor B1 antibody [EPR6457](TA310460); Equilibrium disassociation constant (KD)..
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B1 antibody [EPR6457]; Immunofluorescence analysis of rat oligodendrocytes, staining Eph receptor B1 with TA310460. Cells were fixed with formaldehyde, permeabilized with 0.03% Triton X-100 and blocked with 1% serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/300 in serum + 0.01% Triton X-100) for 10 hours at 4°C. An AlexaFluor?555-conjugated donkey anti-rabbit polyclonal IgG (1/300) was used as the secondary antibody.

 

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