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Anti-EZR Antibody EPR803
|A synthetic peptide corresponding to residues in human Ezrin was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: 1:10000 - 1:50000; IHC-P: 1:250 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC/IF or IP.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Is unsuitable for Flow Cyt,ICC/IF or IP.
|Homo sapiens ezrin (EZR), transcript variant 1|
|CVIL; CVL; HEL-S-105; VIL2|
|Ezrin, a member of the Ezrin, Radixin, Moesin (ERM) family, is a linker protein located between cell surface receptors, adhesion molecules, and actin cytoskeleton (1-2). Ezrin activity is regulated by intramolecular interactions between N- and C-terminal ERM association domains (3). Ezrin tyrosine phosphorylation can also be induced by EGF, PDGF and HGF stimulation. Ezrin interacts with PI3-K protein kinase A and Rho (4). In response to epidermal growth factor (EGF) the microvillar core protein ezrin is phosphorylated transiently to a high level on tyrosine residues in human epidermoid carcinoma A431 cells. Tyr145, lies in the N-terminal region of homology that is common to the band 4.1-talin-ezrin protein family. This tyrosine residue and its vicinal amino acids are conserved throughout the family members, including radixin, moesin, and the two phosphotyrosine phosphatases, PTP H1 and PTP MEG, but not in band 4.1 or talin (5).|
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Western blot - Anti-Ezrin antibody [EPR803]; All lanes : Anti-Ezrin antibody [EPR803] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293Y cell lysate.Lane 3 : A549 cell lysate.Lane 4 : SH-SY5Y cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat Anti-rabbit HRP at 1/2000 dilution.Predicted band size : 69 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EPR803]; Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Ezrin with TA307863 at 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EPR803]; Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma tissue labelling Ezrin with TA307863 at 1/250 dilution.