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Anti-EZR Antibody EP924Y
|A synthetic peptide corresponding to residues surrounding tyrosine 145 of human ezrin was used as an immunogen. This antibody detects both phosphorylated and nonphosphorylated ezrin.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1: 1000~5000; ICC: 1: 100; FC: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens ezrin (EZR), transcript variant 1|
|CVIL; CVL; VIL2|
|Erzin, a member of the Ezrin, Radixin, Moesin (ERM) family, is a linker protein located between cell surface receptors, adhesion molecules, and actin cytoskeleton (1-2). Ezrin activity is regulated by intramolecular interactions between N- and C-terminal ERM association domains (3). Erzin tyrosine phosphorylation can also be induced by EGF, PDGF and HGF stimulation. Erzin interacts with PI3-K protein kinase A and Rho (4). In response to epidermal growth factor (EGF) the microvillar core protein ezrin is phosphorylated transiently to a high level on tyrosine residues in human epidermoid carcinoma A431 cells. Tyr145, lies in the N-terminal region of homology that is common to the band 4.1-talin-ezrin protein family. This tyrosine residue and its vicinal amino acids are conserved throughout the family members, including radixin, moesin, and the two phosphotyrosine phosphatases, PTP H1 and PTP MEG, but not in band 4.1 or talin (5). |
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Western blot - Ezrin antibody [EP924Y]; All lanes : Anti-Ezrin antibody [EP924Y] at 1/10000 dilution.Lane 1 : 293 cell lysate.Lane 2 : A431 cell lysate.Lane 3 : SH-SY5Y cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 69 kDa.Observed band size : 81 kDa .