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Home Antibody All anti-ETF1 antibodies

Anti-ETF1 TRUEMAB Antibody Clone OTI2E3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502891 ETF1 (eRF1) mouse monoclonal antibody, clone OTI2E3 (formerly 2E3) 100ul $379 In Stock
LC401492 ETF1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502891 Carrier-free (BSA/glycerol-free) ETF1 mouse monoclonal antibody, clone OTI2E3 (formerly 2E3) 100ug $450 3-4 weeks

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(2)
FC(2)

OriGene Data

ImmunogenFull length human recombinant protein of human ETF1 (NP_004721) produced in HEK293T cell.
Clone NameClone OTI2E3 IsotypeIgG2a
Species ReactivityHuman Concentration0.97 mg/ml
Guaranteed Application *WB, FC Suggested Dilutions WB 1:500, FLOW 1:100,
Predicted MW Explanation 48.9 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens eukaryotic translation termination factor 1 (ETF1), transcript variant 1
Alternative NameD5S1995; ERF; ERF1; RF1; SUP45L1; TB3-1
Database LinkNP_004721
Entrez Gene 2107 Human
FunctionThis gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in coding and non-coding transcript variants. Pseudogenes of this gene are found on chromosomes 5, 7 and X. [provided by RefSeq, Jan 2012].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETF1 (RC220387 , Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETF1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ETF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Flow cytometric Analysis of Hela cells, using anti-ETF1 antibody(TA502891),(Red), compared to a nonspecific negative control antibody,(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-ETF1 antibody(TA502891),(Red), compared to a nonspecific negative control antibody,(Blue).
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