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Anti-ESR1 Antibody EPR4097

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SKU Description Amount Price Availability*  
TA307749
  • Rabbit monoclonal antibody against ER Alpha(clone EPR4097)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400046) , 20ug Explanation
100ul $325 In Stock
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WB(1)
IHC(8)
Assay(1)

OriGene Data

ImmunogenA recombinant human ER alpha protein was used as an immunogen.
Clone NameEPR4097 IsotypeIgG
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250; ChIP: Use at an assay dependent concentration
Predicted MW Explanation 66 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens estrogen receptor 1 (ESR1), transcript variant 1
Alternative NameER; Era; ESR; ESRA; ESTRR; NR3A1
Database LinkNP_000116
Entrez Gene 2099 Human
FunctionEstrogen receptor alpha (ER alpha) is a ligand-activated transcription factor and member of the steroid hormone receptor family. It possesses highly conserved DNA binding and ligand binding domains for hormone binding, exerting a significant role in activating the transcription of certain genes (1). ER alpha binds to estrogen response elements (ERE) to regulate estrogen-responsive gene expression (2). Ligand-dependent dimerization and phosphorylation both function to regulate the transcriptional activation of ER alpha (3). Phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), regulates ER alpha activity (4). It is a prognostic marker for breast cancer because ER alpha is present in the mammary gland (5).
Related PathwayNuclear Hormone ReceptorTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Estrogen Receptor antibody [EPR4097]; All lanes : Anti-Estrogen Receptor antibody [EPR4097] at 1/1000 dilution.Lane 1 : MCF7 cell lysate.Lane 2 : T47D cell lysate.Lane 3 : HeLa cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 66 kDa.
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ChIP - Anti-Estrogen Receptor antibody [EPR4097]; ChIP analysis using TA307749 binding Estrogen Receptor in MCF7 cells derived from Human breast carcinoma. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with undiluted primary antibody for 16 hours at 4C. Protein binding was detected using real-time PCR. .Positive control: Estrogen treated MCF7 cells tested at PS2 promoter.Negative Control:IgG ChIP and ethanol-depleted cells tested at PS2 promoter
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Estrogen Receptor antibody [EPR4097]; TA307749 at 1/250 dilution staining Estrogen Receptor in Human breast ductal infiltrating carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Estrogen Receptor antibody [EPR4097]; TA307749 at 1/250 dilution staining Estrogen Receptor in Human normal breast by Immunohistochemistry, Paraffin-embedded tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Estrogen Receptor antibody [EPR4097](TA307749); TA307749 showing negative staining in Normal tonsil tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Estrogen Receptor antibody [EPR4097](TA307749); TA307749 showing negative staining in Colonic adenocarcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Estrogen Receptor antibody [EPR4097](TA307749); TA307749 showing positive staining in Endometrial carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Estrogen Receptor antibody [EPR4097](TA307749); TA307749 showing negative staining in Lung adenocarcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Estrogen Receptor antibody [EPR4097](TA307749); TA307749 showing negative staining in Cervical carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor antibody [EPR4097]; TA307749 showing positive staining in Breast ductal infiltrating carcinoma tissue.
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