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Anti-ERG Antibody EPR3863
|A synthetic peptide corresponding to residues on the N-terminus in human ERG was used as an immunogen. This antibody also detects Fli-1.|
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ChIP: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,ICC or IP.
|Homo sapiens v-ets avian erythroblastosis virus E26 oncogene homolog (ERG), transcript variant 2|
|Transcriptional regulator ERG (ETS-related gene) belongs to a family of transcription factors that shares a highly conserved DNA-binding domain (ETS domain) and differs from each other in other domains (such as absence or presence of the Pointed/SAM domain) (1, 2, 3). ERG has been widely studied in human cancers; TMPRSS2-ERG fusion, which occurs on account of oncogenic translocations and interstitial deletions, is implicated in aggressive forms of prostate cancer (4, 5). ERG is linked to normal processes such as mesoderm formation and is found to form functional complexes with Jun/Fos, with the resulting ternary complexes regulating expression of genes such as metalloprotease-1 (MMP-1) and MMP-3 (1). It may also participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of the local chromatin structure (2). Several isoforms exist, which range from the 35-kDa isoform 7 to the 55-kDa isoform ERG-3 (2).|
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Western blot - ERG antibody [EPR3863]; All lanes : Anti-ERG antibody [EPR3863] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HT29 cell lysate.Lane 3 : MCF7 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 55 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ERG antibody [EPR3863]; TA307296 at 1/100 dilution staining ERG in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ERG antibody [EPR3863]; TA307296 at 1/100 dilution staining ERG in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.