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Home Antibody UltraMAB

UltraMAB

ERCC1 UltraMAB Antibody, Clone 2E12

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations (0) Related Products Product Documents
SKU Description Datasheet Price Availability*  
UM500011
  • ERCC1 mouse monoclonal antibody, clone 2E12, 100 ul
$468 In Stock Add to Shopping Cart
UM570011
  • ERCC1 mouse monoclonal antibody, clone 2E12, 30 ul
$120 In Stock Add to Shopping Cart
WB(2)
Data Img
IHC(16)
Data Img
IF(1)
Data Img
10K-CHIP(1)
Data Img
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Also for ERCC1 (NM_001983)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ERCC1 (NP_973730) produced in HEK293T cell.
Clone NameClone 2E12 IsotypeIgG2b
Species ReactivityHuman , Rat , Monkey Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested DilutionsWB 1:500~1000, IHC 1:150, IF 1:100, FLOW 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant 2
Alternative NameCOFS4; RAD10; UV20
Database LinkNP_001974
FunctionThe product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody (Clone 2E12).
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Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-ERCC1 monoclonal antibody (clone 2E12, 1:500)
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Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human tonsil using anti-ERCC1 mouse monoclonal antibody. (Clone 2E12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunofluorescent staining of HeLa cells using ERCC1 mouse monoclonal antibody (UM500011, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
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OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERCC1 mouse monoclonal antibody (Clone 2E12). The positive reactive proteins are highlighted with red arrows in the enlarged subarray. Other positive controls spotted in this subarray are serial dilutions of mouse IgG as controls.
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