Anti-ERCC1 Antibody, clone Clone 4F9

Also for ERCC1 (NM_001983)

Gene Name	Homo sapiens excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence) (ERCC1), transcript variant 2
Synonyms	COFS4; RAD10; UV20
Immunogen	Protein expressed in 293T cell transfected with human ERCC1 expression vector
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	Clone 4F9	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF	Suggested Dilutions	WB 1:2000, IHC 1:50, IF 1:100,
Background	The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.

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WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody (Clone 4F9).

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 4F9, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 4F9, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded lung tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 4F9, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 4F9, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 4F9, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 4F9, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 4F9, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded thyroid tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 4F9, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 4F9, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded prostate tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 4F9, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded bladder tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 4F9, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-ERCC1 mouse monoclonal antibody. (Clone 4F9, Dilution 1:50)

IF Image

Immunofluorescent staining of HeLa cells using anti-ERCC1 mouse monoclonal antibody (UM500008, Clone 4F9).

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