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Home Antibody All anti-ERCC1 antibodies

Anti-ERCC1 Antibody EP2143Y


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA306972 Rabbit monoclonal antibody against ERCC1(clone EP2143Y) 100ul $325 In Stock
LC404356 ERCC1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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Also for ERCC1 (NM_202001)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to N-terminus of human ERCC1 was used. Might also detect heterodimer composed of ERCC1 and XPF/ERCC4 at 100 -150 kDa.
Clone NameEP2143Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:1000 - 1:5000; FC: 1:30
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for ICC,IHC-P or IP.

Reference Data

Target NameHomo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant 1
Alternative NameCOFS4; RAD10; UV20
Database LinkNP_973730
Entrez Gene 2067 Human
FunctionExcision Repair Cross Complementing 1 (ERCC1) is a mammalian nucleotide excision repair (NER) enzyme involved in repair of damaged DNA. ERCC1 is a homologous to RAD10 in Saccharomyces cerevisiae which is required in mitotic intrachromosomal recombination and repair. Specifically, during DNA repair, ERCC1 forms a complex with XPF/ERCC4 which makes incision 5’ to the lesion site (1). ERCC1 is required in repair of cisplatin-induced DNA adducts and ultraviolet (UV)-induced DNA damage. A defect in ERCC1 can lead to Cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) and UV-induced skin cancer (2).
Related PathwayDruggable Genome Nucleotide excision repair

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - ERCC1 antibody [EP2143Y]; Anti-ERCC1 antibody [EP2143Y] at 1/2000 dilution + SKBR-3 cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 32 kDa.Observed band size : 36 kDa .Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.


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