A phospho specific peptide corresponding to residues surrounding Tyrosine 1284 of human HER4/ErbB4 was used as an immunogen. This antibody only detects HER4/ErbB4 phosphorylated at HER4/ErbB4.
Buffer
Store at -20C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
HER4/ErbB4 is a receptor tyrosine kinase that belongs to the human epidermal growth factor receptor (EGFR) family. ErbB4/HER4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum (1). ErbB4/HER4 is activated by neuregulins (NRG), betacellulin (BTC), and heparin-binding EGF-like growth factor (2). Neuregulins regulate the expression of ligand- and voltage-gated channels in neurons and skeletal muscle by the activation of ErbB 1-4. The HER4/ErbB4 receptor is unique among these receptors because its C-terminal tail binds to a protein motif, known as the PDZ domain (3).
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Flow cytometric analysis of permeabilized A431 cells, untreated (green) or EGF-treated (red) using anti-HER4/ErbB4 Phospho (pY1284) RabMAb, and EGF-treated A431 cells using the same antibody preincubated with phospho-HER4/ErbB4 Phospho (pY1284) peptide (blue) or non-phospho-HER4/ErbB4 Phospho (pY1284) peptide (orange)
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