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Home Antibody All anti-ERBB2 antibodies

Anti-ERBB2 PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA323485
  • Anti-ERBB2 (Phospho-Tyr1248) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
IF(1)
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Also for ERBB2 (NM_004448)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of tyrosine1248 (P-E-Y(p)-L-G) derived from Human HER2.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
Predicted MW Explanation 138kDa
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (ERBB2), transcript variant 1
Alternative NameCD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1
Database LinkNP_004439
Entrez Gene 2064 Human
Entrez Gene 13866 Mouse
Entrez Gene 24337 Rat
FunctionThis gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
Related PathwayProtein KinaseTransmembraneDruggable Genome ErbB signaling pathwayCalcium signaling pathwayFocal adhesionAdherens junctionPathways in cancerPancreatic cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 138 kDa . Positive control: MDA cells untreated or treated with EGF lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: MDA cells untreated with EGF lysate Lane2: MDA cells treated with EGF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Nucleus; Cytoplasm; Cell membrane. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using ERBB2 (Phospho-Tyr1248) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
IF Image
Predicted cell location: Nucleus; Cytoplasm; Cell membrane. Positive control: MCF7 cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed MCF7 cells using ERBB2 (Phospho-Tyr1248) antibody at dilution 1/100. (Original magnification:×200)

 

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