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Anti-ERBB2 PHOSPHO Antibody EPR1047
Also for ERBB2 (NM_004448)
|A phosphospecific peptide corresponding to residues surrounding tyrosine 1222 of human HER2 was used as an immungen. This antibody detects HER2 phosphorylated at tyrosine 1222.|
||0.5~1.0 mg/ml (Lot Dependent)
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (ERBB2), transcript variant 1|
|CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1|
|HER2 (ErbB2, epidermal growth factor receptor 2) is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of HER2 can be activated without ligand if it is overexpressed, and by association with other HER proteins (2). Overexpression of HER2 is detected in almost 40% of human breast cancers (3). HER2 is considered one of the major targets for the treatment of breast cancer and other carcinomas. Binding of c-Cbl ubiquitin ligase to Tyr1112 of HER2 leads to poly-ubiquitination of HER2 and enhances its degradation (4). Similar to other growth factors, HER2 exhibits intrinsic protein tyrosine kinase activity and undergoes autophosphorylation (Y1023, Y1248, Y1139, and Y1222). A phosphorylation at tyrosine 1139 has been identified to induce the binding of Src to HER2, leading to HER2 activation of Stat3 alpha (5). |
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Western blot - ErbB 2 (phospho Y1221 + Y1222) antibody [EPR1047]; All lanes : Anti-ErbB 2 (phospho Y1221 + Y1222) antibody [EPR1047] at 1/5000 dilution.Lane 1 : SKBR3 cell lysate was untreated .Lane 2 : SKBR3 cell lysate was treated with Lambda phosphatase.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/1000 dilution.Predicted band size : 138 kDa.