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Anti-EPCAM TRUEMAB Antibody Clone1C1
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for EPCAM (NM_002354)
|Full length human recombinant protein of human EPCAM(NP_002345) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB: 1:400-1:1000, IHC: 1:150-1:500, IF: 1:100, Flow: 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens epithelial cell adhesion molecule (EPCAM)|
|DIAR5; EGP-2; EGP314; EGP40; ESA; HNPCC8; KS1/4; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1|
Entrez Gene 4072 Human
|This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]. |
|ES Cell Differentiation/IPSTransmembrane |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPCAM (RC201989, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPCAM.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPCAM monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)(1:400).
Western blot analysis of extracts (10ug) from 1 cell line by using anti-EPCAM monoclonal antibody at 1:200.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-EPCAM mouse monoclonal antibody at 1:500 dilution. (TA506629)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPCAM mouse monoclonal antibody at 1:500 dilution. (TA506629)
Anti-EPCAM mouse monoclonal antibody (TA506629) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EPCAM(RC201989).
Flow cytometric analysis of live HEK293 cells transfected with EPCAM ORF clone (Cat# RC201989), using anti-EPCAM antibody (TA506629 at 1:100, Red), compared to IgG1 isotype control (Black).
Flow cytometric analysis of live HT29 cells, using anti-EPCAM antibody (TA506629 at 1:100, Red), compared to IgG1 isotype control (Black).
Flow cytometric analysis of live OVCAR-4 cells, using anti-EPCAM antibody (TA506629 at 1:100, Red), compared to IgG1 isotype control (Black).