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Anti-ENO2 Antibody EPR3378


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against Enolase 2 gamma (NSE)(clone EPR3378)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400724) , 20ug Explanation
100ul $325 3-7 Days
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Enolase 2 gamma (NSE) was used as an immunogen.
Clone NameEPR3378 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:1000 - 1:10000; FC: 1:10 - 1:100
Predicted MW Explanation 47 kDa
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for ICC,IHC-P or IP.

Reference Data

Target NameHomo sapiens enolase 2 (gamma, neuronal) (ENO2)
Alternative NameHEL-S-279; NSE
Database LinkNP_001966
Entrez Gene 2026 Human
Entrez Gene 13807 Mouse
Entrez Gene 24334 Rat
FunctionEnolase 2 gamma is a glycolytic enzyme that catalyzes the interconversion of 2-phosphoenolpyruvate to phosphoenolpyruvate in the glycolytic pathway (1, 2). The functional enzyme is a dimer made up of subunits referred to as alpha, beta, and gamma. The alpha-, or nonneuronal, enolase (ENO1) is a nearly ubiquitous form, found in almost all tissues, and its expression precedes that of the other isoforms in the early stage of embryonic development. The beta-, or muscle-specific, enolase (ENO3) is present in adult skeletal muscle, and the gamma-, or neuron-specific, enolase (ENO2) is the major form found in mature neurons and in cells of neuronal origin (2).
Related Pathway Glycolysis / GluconeogenesisMetabolic pathwaysRNA degradation

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* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-NSE antibody [EPR3378]; All lanes : Anti-NSE antibody [EPR3378] at 1/1000 dilution.Lane 1 : Fetal brain lysate.Lane 2 : SH-SY5Y cell lysate.Lane 3 : Y79 cell lysate.Lane 4 : U87-MG cell lysate.Lane 5 : HeLa cell lysate.Lane 6 : HepG2 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 47 kDa.


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