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Home Antibody All anti-ENO2 antibodies

Anti-ENO2 Antibody EPR3377

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307497
  • Rabbit monoclonal antibody against Enolase 2 gamma (NSE) (clone EPR3377 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400724) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for ENO2 (NM_001975)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Enolase 2 gamma (NSE) was used as an immunogen.
Clone NameEPR3377 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:5000 - 1:20000; FC: 1:20 - 1:100; IHC-P: Use at an assay dependent dilution; ICC: 1:100 - 1:250
Predicted MW Explanation 47 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens enolase 2 (gamma, neuronal) (ENO2)
Alternative NameHEL-S-279; NSE
Database LinkNP_001966
Entrez Gene 2026 Human
Entrez Gene 13807 Mouse
Entrez Gene 24334 Rat
FunctionEnolase is a glycolytic enzyme that catalyzes the interconversion of 2-phosphoenolpyruvate to phosphoenolpyruvate in the glycolytic pathway (1, 2). The functional enzyme is a dimer made up of subunits referred to as alpha, beta, and gamma. The alpha-, or nonneuronal, enolase (ENO1) is a nearly ubiquitous form, found in almost all tissues, and its expression precedes that of the other isoforms in the early stage of embryonic development. The beta-, or muscle-specific, enolase (ENO3) is present in adult skeletal muscle, and the gamma-, or neuron-specific, enolase (ENO2) is the major form found in mature neurons and in cells of neuronal origin (2).
Related Pathway Glycolysis / GluconeogenesisMetabolic pathwaysRNA degradation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - NSE antibody [EPR3377]; All lanes : Anti-NSE antibody [EPR3377] at 1/20000 dilution.Lane 1 : fetal brain lysate.Lane 2 : SH-SY-5Y lysate.Lane 3 : HeLa lysate.Lane 4 : Y79 lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 47 kDa.Observed band size : 47 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NSE antibody [EPR3377]; Immunohistochemical analysis of paraffin-embedded human brain tissue using 1/250-1/500 TA307497.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody [EPR3377]; ICC/IF image of TA307497
FC Image
Flow Cytometry-NSE antibody [EPR3377](TA307497); Overlay histogram showing HeLa cells stained with TA307497 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized with 0.1% PBS-Tween 20 used under the same conditions.

 

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