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Home Antibody All anti-ELANE antibodies

Anti-ELANE Antibody EPR7479

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310453
  • Rabbit Monoclonal antibody against Neutrophil Elastase (ELANE)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC419618) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
FC(1)
Assay(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues at the C-terminus of human Neutrophil Elastase was used as an immunogen.
Clone NameEPR7479 IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ASSAY, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; FC: 1:10 - 1:100; ICC/IF: 1:250 - 1:500
BufferDoes not react with Mouse, Rat. Is unsuitable for IP.
Purification pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens elastase, neutrophil expressed (ELANE)
Alternative NameELA2; GE; HLE; HNE; NE; PMN-E; SCN1
Database LinkNP_001963
FunctionNeutrophil Elastase hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. Neutrophil Elastase degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this protein are associated with cyclic neutropenia and severe congenital neutropenia (SCN) (1).
Related Pathway

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WB Image
Western blot - Anti-Neutrophil Elastase antibody [EPR7479]; Anti-Neutrophil Elastase antibody [EPR7479] at 1/1000 dilution + HL60 cell lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 29 kDa.
Assay Image
Other-Anti-Neutrophil Elastase antibody [EPR7479](TA310453); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody [EPR7479]; Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labelling Neutrophil Elastase with TA310453 at 1/250 dilution.
FC Image
Flow Cytometry - Anti-Neutrophil Elastase antibody [EPR7479]; Overlay histogram showing HL60 cells stained with TA310453 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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