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Anti-EIF5A Antibody EP527Y
Also for EIF5A (NM_001970)
|A synthetic peptide corresponding to residues near the C-term of human eIF-5A was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:1000; IHC-P: Use at an assay dependent dilution; FC: 1:80
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for ICC or IP.
|Homo sapiens eukaryotic translation initiation factor 5A (EIF5A), transcript variant B|
|EIF-5A; EIF5A1; eIF5AI|
Entrez Gene 1984 Human
Entrez Gene 276770 Mouse
Entrez Gene 287444 Rat
|The human eukaryotic translation initiation factor 5A (eIF-5A) is an 18 kDa protein (1). eIF-5A precursor [(ec-eIF-5A(lys)] is the only cellular protein known to contain a specific lysine residue which is transformed into the unique amino acid hypusine [N-(4-amino-2-hydroxybutyl)-lysine] by a series of post-translational reactions (2). eIF-5A promotes the formation of the first peptide bond during the initial stage of protein synthesis (3).|
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Western blot - eIF5A antibody [EP527Y]; Anti-eIF5A antibody [EP527Y] at 1/1000 dilution + Jurkat cell lysate.Predicted band size : 18 kDa.Observed band size : 18 kDa.
Immunohistochemistry (Paraffin-embedded sections) - eIF5A antibody [EP527Y]; Ab32407, at a dilution of 1/250, staining eIF5A in paraffin embedded human urinary bladder carcinoma tissue sections by Immunohistochemistry.
Flow Cytometry-Anti-eIF5A antibody [EP527Y](TA300560); Overlay histogram showing Jurkat cells stained with TA300560 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.