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Anti-EIF5A Antibody EP526Y
Also for EIF5A (NM_001970)
|A synthetic peptide corresponding to residues near the C-term of human eIF-5A was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||IHC-FoFr: 1:400; WB: 1:5000 - 1:10000; IHC-P: Use at an assay dependent dilution; FC: 1:50 - 1:80; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Is unsuitable for ICC.
|Homo sapiens eukaryotic translation initiation factor 5A (EIF5A), transcript variant B|
|EIF-5A; EIF5A1; eIF5AI|
|The human eukaryotic translation initiation factor 5A (eIF-5A) is an 18 kDa protein (1). eIF-5A precursor [(ec-eIF-5A(lys)] is the only cellular protein known to contain a specific lysine residue which is transformed into the unique amino acid hypusine [N-(4-amino-2-hydroxybutyl)-lysine] by a series of post-translational reactions (2). eIF-5A promotes the formation of the first peptide bond during the initial stage of protein synthesis (3).|
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Western blot - eIF5A antibody [EP526Y]; Anti-eIF5A antibody [EP526Y] at 1/10000 dilution + Jurkat cell lysate.Predicted band size : 18 kDa.Observed band size : 18 kDa.
Immunohistochemistry (Paraffin-embedded sections) - eIF5A antibody [EP526Y]; ab32443 at a 1:250 dilution staining eIF5A in human adenocarcinoma of uterus using Immunohistochemistry, Paraffin Embedded Tissue.
Flow Cytometry-eIF5A antibody [EP526Y](ab32443); Overlay histogram showing Jurkat cells stained with ab32443 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.