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Home Antibody All anti-EIF5A antibodies

Anti-EIF5A Antibody EP526Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300557
  • Rabbit Monoclonal Antibody against EIF5A (Clone EP526Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419616) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for EIF5A (NM_001970)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-term of human eIF-5A was used as immunogen.
Clone NameEP526Y IsotypeIgG
Species ReactivityMouse, Rat, Human, Fruit fly (Drosophila melanogaster) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsIHC-FoFr: 1:400; WB: 1:5000 - 1:10000; IHC-P: Use at an assay dependent dilution; FC: 1:50 - 1:80; IP: 1:50
Predicted MW Explanation 18 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction
Note Is unsuitable for ICC.

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 5A (EIF5A), transcript variant B
Alternative NameEIF-5A; EIF5A1; eIF5AI
Database LinkNP_001961
Entrez Gene 1984 Human
Entrez Gene 276770 Mouse
Entrez Gene 287444 Rat
FunctionThe human eukaryotic translation initiation factor 5A (eIF-5A) is an 18 kDa protein (1). eIF-5A precursor [(ec-eIF-5A(lys)] is the only cellular protein known to contain a specific lysine residue which is transformed into the unique amino acid hypusine [N-(4-amino-2-hydroxybutyl)-lysine] by a series of post-translational reactions (2). eIF-5A promotes the formation of the first peptide bond during the initial stage of protein synthesis (3).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - eIF5A antibody [EP526Y]; Anti-eIF5A antibody [EP526Y] at 1/10000 dilution + Jurkat cell lysate.Predicted band size : 18 kDa.Observed band size : 18 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - eIF5A antibody [EP526Y]; TA300557 at a 1:250 dilution staining eIF5A in human adenocarcinoma of uterus using Immunohistochemistry, Paraffin Embedded Tissue.
FC Image
Flow Cytometry-eIF5A antibody [EP526Y](TA300557); Overlay histogram showing Jurkat cells stained with TA300557 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

 

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