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Home Antibody All anti-EIF4EBP1 antibodies

Anti-EIF4EBP1 PHOSPHO Antibody EPR2169Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301188
  • Rabbit Monoclonal Antibody against EIF4EBP1 (Clone EPR2169Y) (Phospho-specific)
100ul 325 3-7 Days
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WB(1)
IHC(1)
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Also for EIF4EBP1 (NM_004095)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding threonine 45 was used as an immunogen. This antibody detects 4E-BP1/2/3 phosophorylated at threonine 45.
Clone NameEPR2169Y IsotypeIgG
Species ReactivityHuman, Mouse Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:5000; IHC: 1:50; ICC: 1: 100; IP: 1: 10
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Does not react with Rat

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1)
Alternative Name4E-BP1; 4EBP1; BP-1; PHAS-I
Database LinkNP_004086
Function4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4)
Related Pathway

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WB Image
Western blot - eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y]; All lanes : Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y].Lane 1 : 293T cell lysate, untreated.Lane 2 : 293T cell lysate, treated with FBS.Lysates/proteins at 10 µg per lane.Secondary.HRP conjugated Goat anti-rabbit at 1/2000 dilution.Predicted band size : 13 kDa.Observed band size : 14-19 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y]; Human breast carcinoma tissue stained with TA301188 at 1/50 dilution.

 

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