Homo sapiens eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1)
Synonyms:
4E-BP1; 4EBP1; BP-1; PHAS-I
Immunogen
A phospho specific peptide corresponding to residues surrounding threonine 45 was used as an immunogen. This antibody detects 4E-BP1/2/3 phosophorylated at threonine 45.
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4)
Related Pathway
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Western blot analysis on 293T cell lysates using anti-Phospho-4E-Bp1/2/3 (pT45), 1:10,000 dilution. Cells were either (A) untreated (B) treated with FBS.
