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Anti-EIF4EBP1 PHOSPHO Antibody EPR654(2)A
Also for EIF4EBP1 (NM_004095)
|A phospho specific peptide corresponding to residues surrounding threonine 70 was used as an immunogen. This antibody detects 4E-BP1 phosophorylated at threonine 70.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500; IHC: 1: 100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1)|
|4E-BP1; 4EBP1; BP-1; PHAS-I|
|4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 70 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4) |
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Western blot - eIF4EBP1 (phospho T70) antibody [EPR654(2)AY]; All lanes : Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] at 1/500 dilution.Lane 1 : 293T cell lysates un-treated.Lane 2 : 293T cell lysates treated with FBS.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 13 kDa.Observed band size : 17 kDa .