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Anti-EIF4EBP1 Antibody Y329
Also for EIF4EBP1 (NM_004095)
|A synthetic peptide corresponding to residues near the N-term of human4E-BP1 was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:5000 - 1:10000; IHC-P: Use at an assay dependent concentration; ICC: 1:100 - 1:250; FC: 1:50 - 1:100; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Homo sapiens eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1)|
|4E-BP1; 4EBP1; BP-1; PHAS-I|
|4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5’ cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4) |
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Western blot - eIF4EBP1 antibody [Y329]; Anti-eIF4EBP1 antibody [Y329] at 1/10000 dilution + 293 cell lysate.Predicted band size : 13 kDa.Observed band size : 15 - 20 kDa .
Immunohistochemistry (Paraffin-embedded sections) - eIF4EBP1 antibody [Y329]; ab32024, at a 1/100 dilution, staining human eIF4EBP1 in colon carcinoma by Immunohistochemistry, Paraffin embedded tissue
Flow Cytometry - Anti-eIF4EBP1 antibody [Y329]; Overlay histogram showing HT1080 cells stained with ab32024 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HT1080 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.