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Home Antibody All anti-EIF4EBP1 antibodies

Anti-EIF4EBP1 Antibody Y329

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300488
  • Rabbit Monoclonal Antibody against EIF4EBP1 (Clone Y329)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401322) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for EIF4EBP1 (NM_004095)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-term of human4E-BP1 was used as immunogen.
Clone NameY329 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:5000 - 1:10000; IHC-P: Use at an assay dependent concentration; ICC: 1:100 - 1:250; FC: 1:50 - 1:100; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1)
Alternative Name4E-BP1; 4EBP1; BP-1; PHAS-I
Database LinkNP_004086
Entrez Gene 1978 Human
Entrez Gene 13685 Mouse
Entrez Gene 116636 Rat
Function4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5’ cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4)
Related Pathway ErbB signaling pathwaymTOR signaling pathwayInsulin signaling pathwayAcute myeloid leukemia

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - eIF4EBP1 antibody [Y329]; Anti-eIF4EBP1 antibody [Y329] at 1/10000 dilution + 293 cell lysate.Predicted band size : 13 kDa.Observed band size : 15 - 20 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - eIF4EBP1 antibody [Y329]; TA300488, at a 1/100 dilution, staining human eIF4EBP1 in colon carcinoma by Immunohistochemistry, Paraffin embedded tissue
FC Image
Flow Cytometry - Anti-eIF4EBP1 antibody [Y329]; Overlay histogram showing HT1080 cells stained with TA300488 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HT1080 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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