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Home Antibody All anti-EIF4A1 antibodies

Anti-EIF4A1 Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA302076 Rabbit Polyclonal Antibody against EIF4A1 (N-term) 100ug $325 In Stock
LC400547 EIF4A1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(2)
IHC(1)
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Also for EIF4A1 (NM_001416)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenThis EIF4A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EIF4A1.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman (Predicted: Mouse, Bovine, Monkey) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000, IHC: 1:10~50
Predicted MW Explanation 46154 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 4A1 (EIF4A1), transcript variant 1
Alternative NameDDX2A; EIF-4A; eIF-4A-I; EIF4A; eIF4A-I
Database LinkNP_001407
Entrez Gene 1973 Human
Entrez Gene 13681 Mouse
Entrez Gene 695050 Monkey
FunctionEIF4A1 is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of anti-EIF4A1 Pab (Cat. #TA302076) in K562 cell line lysates (35ug/lane). EIF4A1(arrow) was detected using the purified Pab.
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Western blot analysis of EIF4A1 (arrow) using rabbit polyclonal EIF4A1 Antibody (N-term) (Cat.#TA302076). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4A1 gene (Lane 2) (Origene Technologies).
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EIF4A1 antibody (N-term) (Cat.#TA302076), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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