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Home Antibody All anti-EIF3E antibodies

Anti-EIF3E Antibody EPR6877

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310449
  • Rabbit Monoclonal antibody against eIF-3E (EIF3E)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400602) , 20ug
100ul 325 3-7 Days
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WB(1)
Assay(1)
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Also for EIF3E (NM_001568)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human eIF-3E was used as an immunogen.
Clone NameEPR6877 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ASSAY Suggested DilutionsWB: 1:1000 - 1:10000; ICC/IF: 1:100 - 1:250
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,IHC-P or IP.

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 3, subunit E (EIF3E)
Alternative NameeIF3-p46; EIF3-P48; EIF3S6; INT6
Database LinkNP_001559
FunctioneIF-3E is the p48 subunit of the translational initiation factor eIF3. It forms a multi-factor complex with eIF1, eIF5, and the eIF2-GTP-Met-tRNA ternary complex that binds to the 40S ribosomal subunit to form the 43S preinitiation complex (PIC). eIF-3E also prevents the premature joining of the 60S ribosomal subunit until the start codon is recognized. In addition, eIF-3E plays a role in recruiting eIF4G-associated mRNAs to the 43S PIC to form the 48S PIC and initiate mRNA scanning (1). It is required for nonsense-mediated mRNA decay (NMD) and acts in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. eIF-3E also interacts with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins (2).
Related Pathway

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WB Image
Western blot - Anti-eIF3e antibody [EPR6877]; All lanes : Anti-eIF3e antibody [EPR6877] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : 293T cell lysate.Lane 3 : BxPC3 cell lysate.Lane 4 : A431 cell lysate.Lane 5 : Saos 2 cell lysate.Lane 6 : Jurkat cell lysate.Lane 7 : NIH 3T3 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti rabbit at 1/2000 dilution.Predicted band size : 52 kDa.Observed band size : 48 kDa .
Assay Image
Other-Anti-eIF3e antibody [EPR6877](TA310449); Equilibrium disassociation constant (KD)..

 

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