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Home Antibody All anti-EIF2S1 antibodies

Anti-EIF2S1 TRUEMAB Antibody Clone OTI1H1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA501402 EIF2S1 mouse monoclonal antibody, clone OTI1H1 (formerly 1H1) 100ul $325 In Stock
LC401321 EIF2S1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501402 Carrier-free (BSA/glycerol-free) EIF2S1 mouse monoclonal antibody, clone OTI1H1 (formerly 1H1) 100ug $450 3-4 weeks
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WB(2)
IHC(1)
FC(1)
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Also for EIF2S1 (NM_004094)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell.
Clone NameClone OTI1H1 IsotypeIgG1
Species ReactivityHuman Concentration0.51 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:2000, IHC 1:150, FLOW 1:100
Predicted MW Explanation 35.9 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa (EIF2S1)
Alternative NameEIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A
Database LinkNP_004085
Entrez Gene 1965 Human
FunctionThe translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (RC200368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF2S1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-EIF2S1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501402)
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Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody(TA501402),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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