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Home Antibody All anti-EIF2S1 antibodies

Anti-EIF2S1 TRUEMAB Antibody clone 3H4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501313
  • anti-EIF2S1 mouse monoclonal antibody, clone 3H4
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401321) , 20ug
100ul 379
$284
In Stock
CF501313
  • Carrier-free (BSA/glycerol-free) EIF2S1 mouse monoclonal antibody, clone 3H4
100ug $450 3-4 weeks
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WB(2)
IHC(5)
IF(1)
FC(2)
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Also for EIF2S1 (NM_004094)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell.
Clone Nameclone 3H4 IsotypeIgG1
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.69 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa (EIF2S1)
Alternative NameEIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A
Database LinkNP_004085
FunctionThe translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (RC200368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF2S1 monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-EIF2S1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501313, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-EIF2S1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501313, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-EIF2S1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501313, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-EIF2S1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501313, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-EIF2S1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501313, Dilution 1:50)
IF Image
Anti-EIF2S1 mouse monoclonal antibody (TA501313) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EIF2S1(RC200368).
FC Image
HEK293T cells transfected with either RC200368 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-EIF2S1 antibody(TA501313), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody(TA501313),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

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