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Anti-EIF2S1 PHOSPHO Antibody E90
Also for EIF2S1 (NM_004094)
|A synthetic phospho-peptide corresponding to residues surrounding Ser51 of human eIF-2a was used as immunogen. The antibody only detects eIF-2a phosphorylated on Serine 51. Predicted to cross-react with mouse, rat, bovine and pig, based on sequence homology.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:1,000-10,000; IHC 1:50-1:100; ICC: 1:100-1:250;
|Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.|
|Homo sapiens eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa (EIF2S1)|
|EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A|
|Eukaryotic initiation factor 2 (eIF2) plays a central role in initiating translation, and provides for a rate-limiting step in protein synthesis. Phosphorylation of a-subunit of eIF2 effectively prevents formation of the eIF2/GTP/Met-tRNAi complex and inhibits global protein synthesis (1-3). Three distinct protein kinases regulate protein synthesis in eukaryotic cells by phosphorylating the a-subunit of eIF2 at Serine51 (3). Phosphorylation occurs under a wide variety of different stimuli, including heat shock, serum deprivation, glucose starvation, amino acid starvation, exposure to heavy metal ions, and viral infection (3).|
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Anti-EIF2S1 (phospho S51) [E90] antibody (TA310445) at 1/500 dilution + PC12 cell lysate
Immunohistochemical analysis of paraffin-embedded human liver carcinoma using TA310445 at 1/50 dilution.Immunohistochemical analysis of paraffin-embedded human liver carcinoma using TA310445 at 1/50 dilution.
TA310445 showing positive staining in Breast carcinoma tissue.
TA310445 showing positive staining in Cervical carcinoma tissue.
TA310445 showing positive staining in Colonic adenocarcinoma tissue.
TA310445 showing positive staining in Hepatocellular carcinoma tissue.
TA310445 at 1/100 staining human epithelial cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA before incubation with the antibody for 1 hour. An Alexa-Fluor 647 conjugated goat anti-rabbit IgG was used as the secondary.
Overlay histogram showing HeLa cells stained with TA310445 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (TA310445, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.