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Home Antibody All anti-EIF2B3 antibodies

Anti-EIF2B3 TRUEMAB Antibody Clone OTI1A4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA503758 EIF2B3 mouse monoclonal antibody, clone OTI1A4 (formerly 1A4) 100ul $379 In Stock
LC412535 EIF2B3 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503758 Carrier-free (BSA/glycerol-free) EIF2B3 mouse monoclonal antibody, clone OTI1A4 (formerly 1A4) 100ug $450 3-4 weeks
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WB(2)
IHC(5)
IF(1)
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Also for EIF2B3 (NM_020365)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human EIF2B3(NP_065098) produced in HEK293T cell.
Clone NameClone OTI1A4 IsotypeIgG1
Species ReactivityHuman, Dog Concentration0.7 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100,
Predicted MW Explanation 50.1 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa (EIF2B3), transcript variant 1
Alternative NameEIF-2B; EIF2Bgamma
Database LinkNP_065098
Entrez Gene 8891 Human
Entrez Gene 475379 Dog
FunctionThe protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2B3 (RC223570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2B3.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF2B3 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-EIF2B3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503758)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-EIF2B3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503758)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-EIF2B3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503758)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-EIF2B3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503758)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-EIF2B3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503758)
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Anti-EIF2B3 mouse monoclonal antibody (TA503758) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EIF2B3(RC223570).
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