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Home Antibody All anti-GCN2 antibodies

Anti-GCN2 Antibody EPR5970(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310526
  • Rabbit Monoclonal antibody against GCN2
100ul $325 3-7 Days
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WB(1)
IHC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human GCN2 was used as an immunogen.
Clone NameEPR5970(2) IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:250 - 1:500; FC: 1:100 - 1:500
BufferDoes not react with Mouse, Rat. Is unsuitable for IP.
Purification pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4)
Alternative NameGCN2; PVOD2
Database LinkNP_001013725
FunctionIn eukaryotic cells, protein synthesis is regulated in response to various environmental stresses by phosphorylating the alpha subunit of the eukaryotic initiation factor 2 (eIF2alpha). Four different eIF2alpha kinases have been identified in mammalian cells, the heme-regulated inhibitor (HRI), the interferon-inducible RNA-dependent kinase (PKR) and the endoplasmic reticulum-resident kinase (PERK) and GCN2, which was previously characterized from Saccharomyces cerevisiae, Drosophila melanogaster and Neurospora crassa. GCN2 is the unique eIF2alpha kinase present in all eukaryotes from yeast to mammals (1). GCN2, a sensor of amino acid deficiency, plays a key role in yeast and mammals in modulating amino acid metabolism as part of adaptation to nutrient deprivation. (2). Results demonstrate that PERK and GCN2 function to cooperatively regulate eIF2alpha phosphorylation and cyclin D1 translation after unfolded protein response pathway activation (3).
Related Pathway

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WB Image
Western blot - Anti-GCN2 antibody [EPR5970(2)]; All lanes : Anti-GCN2 antibody [EPR5970(2)] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lane 3 : MOLT4 cell lysate.Lane 4 : A549 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 187 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCN2 antibody [EPR5970(2)]; Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling GCN2 with TA310526 at 1/100 dilution.

 

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