Home Antibody All anti-EIF2AK4 antibodies
Also for EIF2AK4 (NM_001013703)
|This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region with phenylalanine at position 1116 of human GCN2.|
||Lot dependent; please refer to CoA along with shipment
||WB: 1: 100~500; IHC: 1:50~100; ELISA: 1: 1,000;
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4)|
|EIF2AK4 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2S1; MIM 603907) to downregulate protein synthesis in response to varied cellular stresses (Berlanga et al., 1999 [PubMed 10504407]).[supplied by OMIM]|
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(LEFT)The anti-GCN2 Pab is used in Western blot to detect GCN2 in 293 cell lysate (Lane 1) and NIH/3T3 cell lysate (Lane 2). (RIGHT)Western blot analysis of GCN2 (arrow) using GCN2 Antibody (Center F1116) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GCN2 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.