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Anti-EIF2AK4 PHOSPHO Antibody EPR2320Y
Also for EIF2AK4 (NM_001013703)
|A phospho specific peptide corresponding to residues surrounding threonine 899 of human GCN2 was used as an immunogen. This antibody detects GCN2 phosphorylyated at threonine 899.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:2000
|pH: 7.20Preservative: 0.01% Sodium azideConstituents: 59% PBS, 40% Glycerol, 0.05% BSA|
|Protein A purified
|Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
|Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4)|
Entrez Gene 440275 Human
|In eukaryotic cells, protein synthesis is regulated in response to various environmental stresses by phosphorylating the alpha subunit of the eukaryotic initiation factor 2 (eIF2alpha). Four different eIF2alpha kinases have been identified in mammalian cells, the heme-regulated inhibitor (HRI), the interferon-inducible RNA-dependent kinase (PKR) and the endoplasmic reticulum-resident kinase (PERK) and GCN2, which was previously characterized from Saccharomyces cerevisiae, Drosophila melanogaster and Neurospora crassa. GCN2 is the unique eIF2alpha kinase present in all eukaryotes from yeast to mammals (1). GCN2, a sensor of amino acid deficiency, plays a key role in yeast and mammals in modulating amino acid metabolism as part of adaptation to nutrient deprivation. (2). Results demonstrate that PERK and GCN2 function to cooperatively regulate eIF2alpha phosphorylation and cyclin D1 translation after unfolded protein response pathway activation (3). |
|Protein KinaseDruggable Genome |
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Western blot - GCN2 (phospho T899) antibody [EPR2320Y]; All lanes : Anti-GCN2 (phospho T899) antibody [EPR2320Y] at 1/2000 dilution.Lane 1 : Hela cell lysates un-treated.Lane 2 : Hela cell lysates treated with calyculin A.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 187 kDa.Observed band size : 220 kDa .