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Home Antibody All anti-EIF2AK4 antibodies

Anti-EIF2AK4 PHOSPHO Antibody EPR2319Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301169
  • Rabbit Monoclonal Antibody against EIF2AK4 (Clone EPR2319Y) (Phospho-specific)
100ul 325 3-7 Days
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WB(1)
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Also for EIF2AK4 (NM_001013703)
cDNA Clone shRNA/siRNA Primer Pair Protein Request Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding threonine 667 of human GCN2 was used as an immunogen. This antibody detects GCN2 phosphorylyated at threonine 667.
Clone NameEPR2319Y IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:1000 - 1:2000; IP: 1:30
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4)
Alternative NameGCN2; PVOD2
Database LinkNP_001013725
Entrez Gene 440275 Human
FunctionIn eukaryotic cells, protein synthesis is regulated in response to various environmental stresses by phosphorylating the alpha subunit of the eukaryotic initiation factor 2 (eIF2alpha). Four different eIF2alpha kinases have been identified in mammalian cells, the heme-regulated inhibitor (HRI), the interferon-inducible RNA-dependent kinase (PKR) and the endoplasmic reticulum-resident kinase (PERK) and GCN2, which was previously characterized from Saccharomyces cerevisiae, Drosophila melanogaster and Neurospora crassa. GCN2 is the unique eIF2alpha kinase present in all eukaryotes from yeast to mammals (1). GCN2, a sensor of amino acid deficiency, plays a key role in yeast and mammals in modulating amino acid metabolism as part of adaptation to nutrient deprivation. The role of GCN2 in adaptation to long-term amino acid deprivation in mammals, however, is poorly understood. GCN2-deficient mice developed liver steatosis and exhibited reduced lipid mobilization. Liver steatosis in Gcn2(-/-) mice was found to be caused by unrepressed expression of lipogenic genes, including Srebp-1c and Fa (2). Results demonstrate that PERK and GCN2 function to cooperatively regulate eIF2alpha phosphorylation and cyclin D1 translation after unfolded protein response pathway activation (3).
Related PathwayProtein KinaseDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - GCN2 (phospho T667) antibody [EPR2319Y]; All lanes : Anti-GCN2 (phospho T667) antibody [EPR2319Y] at 1/2000 dilution.Lane 1 : HeLa cell lysate - untreated.Lane 2 : HeLa cell lysate - treated with LP.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution.Predicted band size : 220 kDa.Observed band size : 220 kDa.Additional bands at : 180 kDa. We are unsure as to the identity of these extra bands.

 

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