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Anti-EIF2AK4 PHOSPHO Antibody EPR2319Y
Also for EIF2AK4 (NM_001013703)
|A phospho specific peptide corresponding to residues surrounding threonine 667 of human GCN2 was used as an immunogen. This antibody detects GCN2 phosphorylyated at threonine 667.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:2000; IP: 1:30
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC/IF or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
|Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4)|
Entrez Gene 440275 Human
|In eukaryotic cells, protein synthesis is regulated in response to various environmental stresses by phosphorylating the alpha subunit of the eukaryotic initiation factor 2 (eIF2alpha). Four different eIF2alpha kinases have been identified in mammalian cells, the heme-regulated inhibitor (HRI), the interferon-inducible RNA-dependent kinase (PKR) and the endoplasmic reticulum-resident kinase (PERK) and GCN2, which was previously characterized from Saccharomyces cerevisiae, Drosophila melanogaster and Neurospora crassa. GCN2 is the unique eIF2alpha kinase present in all eukaryotes from yeast to mammals (1). GCN2, a sensor of amino acid deficiency, plays a key role in yeast and mammals in modulating amino acid metabolism as part of adaptation to nutrient deprivation. The role of GCN2 in adaptation to long-term amino acid deprivation in mammals, however, is poorly understood. GCN2-deficient mice developed liver steatosis and exhibited reduced lipid mobilization. Liver steatosis in Gcn2(-/-) mice was found to be caused by unrepressed expression of lipogenic genes, including Srebp-1c and Fa (2). Results demonstrate that PERK and GCN2 function to cooperatively regulate eIF2alpha phosphorylation and cyclin D1 translation after unfolded protein response pathway activation (3). |
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Western blot - GCN2 (phospho T667) antibody [EPR2319Y]; All lanes : Anti-GCN2 (phospho T667) antibody [EPR2319Y] at 1/2000 dilution.Lane 1 : HeLa cell lysate - untreated.Lane 2 : HeLa cell lysate - treated with LP.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution.Predicted band size : 220 kDa.Observed band size : 220 kDa.Additional bands at : 180 kDa. We are unsure as to the identity of these extra bands.