Home Antibody All anti-EIF2AK3 antibodies
Also for EIF2AK3 (NM_010121)
|This whole rabbit serum was prepared by repeated immunizations with a recombinant fusion protein from amino acids 601-1115 of mouse deltaN PERK (see link below for the full length sequence of the mouse gene product).|
||ELISA: 1:4,000 - 1:20,000, WB: 1:500 – 1:3000, IF: User Optimized, IP: 10-30ul
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|The PKR-like endoplasmic reticulum kinase (PERK, also know as Eukaryotic translation initiation factor 2-alpha kinase 3) is a type I transmembrane protein localized to the endoplasmic reticulum (ER). PERK consists of an N-terminal ER luminal domain, a membrane-spanning region, and a cytosolic C-terminal serine/threonine kinase domain (1). The luminal domain of PERK is bound to the ER chaperone GRP78 in unstressed cells (2). PERK activation occurs upon accumulation of misfolded proteins and the ER lumen, which triggers GRP78 dissociation from PERK thereby allowing PERK dimerization and autophosphorylation (3, 4). PERK phosphorylates two established targets: the eukaryotic translation initiation factor 2 alpha (eIF2?, (1)) and the Nrf2 transcription factor (5). Phosphorylation of eIF2? results in attenuation of translation initiation (6). The translational block also contributes to cell cycle arrest due to loss of the G1 regulatory protein, cyclin D1 (7). PERK-dependent phosphorylation of Nrf2 promotes transcription of phase II detoxifying enzymes which is critically important for elimination of intracellular reactive oxygen species (8). Thus, while inhibiting new protein synthesis and thereby decreasing the ER protein load PERK simultaneously induces expression of genes that help restore cellular redox homeostasis and promote survival.
|Mus musculus eukaryotic translation initiation factor 2 alpha kinase 3 (Eif2ak3)|
|AI427929; Pek; Perk|
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Western blot analysis using Immuno-chemical's anti-PERK to detect PERK in cell lysates. 300ug PERK over-expressing 293T cell lysate (lanes 1 & 2), or 800ug wild type (Lanes 3 & 4), and PERK knock out (lanes 5 & 6) MEF cell lysate were immunoprecipated with 15ul anti-PERK, followed by western bloting with 1:1000 dilution of anti-PERK in 5% milk/TBST buffer. Lane 1, 293T cells over-expressing Myc-PERK wt, Lane 2 , 293T cells over-expressing Myc-PERK K618A. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.
Immunohistochemistry staining of mouse mammary gland samples from lactating mice (L10) with Immunochemical’s anti-PERK. Positive staining signal observed in wild type mouse sample with anti-PERK staining only (middle image), but not in the knock out mouse sample (right image) and pre-immune serum staining (left image) The anti-PERK was diluted 1:1,000 in 5% goat serum in PBS and allowed to incubate for 2h at room temperature in a humidified chamber. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.