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Home Antibody All anti-EIF2AK2 antibodies

Anti-EIF2AK2 PHOSPHO Antibody EPR2152Y

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SKU Description Amount Price Availability*  
TA301141
  • Rabbit Monoclonal Antibody against EIF2AK2 (Thr446,Clone EPR2152Y) (Phospho-specific)
100ul $325 3-7 Days
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WB(1)

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Thr451 of human PKR (Interferon-inducible RNA-dependent protein kinase) was used as immunogen. The antibody only detects PKR phosphorylated on Threonine 446.
Clone NameEPR2152Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: Use at an assay dependent dilution
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2), transcript variant 1
Alternative NameEIF2AK1; PKR; PPP1R83; PRKR
Database LinkNP_002750
Entrez Gene 5610 Human
FunctionProtein kinase R (PKR) is a serine/threonine kinase that is induced by interferon and activated by double-stranded RNA. Upon activation, PKR is autophosphorylated at multiple sites and phosphorylates the ?-subunit of the eukaryotic initiation factor-2 eIF2 (eIF2?), inhibiting protein synthesis (1,2). PKR controls the activation of several transcription factors (1) and mediates the effects of interferon via its capacity to inhibit protein synthesis (2). It has been shown that the autophosphorylation sites Thr-446 and Thr-451 in the activation loop of Human PKR are critical for its kinase activity (3).
Related PathwayProtein KinaseTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - PKR (phospho T451) antibody [EPR2152Y]; All lanes : Anti-PKR (phospho T451) antibody [EPR2152Y] at 1/2000 dilution.Lane 1 : HeLa cell lysate un-treated.Lane 2 : HeLa cell lysate treated with IFN-alpha.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 62 kDa.Observed band size : 68 kDa .
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