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Anti-EIF2AK2 Antibody YE350
Also for EIF2AK2 (NM_002759)
|A synthetic peptide corresponding to residues following the first double-stranded RNA-binding domain of human PKR was used as immunogen. The antibody does not cross-react with other GCN2 family members.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1: 1,000-5,000; IHC: 1:250-500; ICC: 1:250-500; IP: 1: 100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Does not react with Mouse, Rat
|Homo sapiens eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2), transcript variant 1|
|EIF2AK1; PKR; PPP1R83; PRKR|
|Protein kinase R (PKR) is a serine/threonine kinase that is induced by interferon and activated by double-stranded RNA. Upon activation, PKR is autophosphorylated at multiple sites and phosphorylates the a-subunit of the eukaryotic initiation factor-2 eIF2 (eIF2a), inhibiting protein synthesis (1,2). PKR controls the activation of several transcription factors (1) and mediates the effects of interferon via its capacity to inhibit protein synthesis (2). It has been shown that the autophosphorylation sites Thr-446 and Thr-451 in the activation loop of Human PKR are critical for its kinase activity (3). |
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Western blot - PKR antibody [YE350]; Anti-PKR antibody [YE350] at 1/5000 dilution + HeLa cell lysate.Predicted band size : 62 kDa.Observed band size : 68 kDa .
Immunohistochemistry (Paraffin-embedded sections) - PKR antibody [YE350]; Immunohistochemical analysis of paraffin-embedded human stomach carcinoma using TA300449 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - PKR antibody [YE350]; Immunofluorescent staining of HeLa cells using TA300449 at 1/250 dilution.