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Home Antibody All anti-EHD1 antibodies

Anti-EHD1 Antibody EPR4954

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307835
  • Rabbit monoclonal antibody against EHD1(clone EPR4954)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC416419) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(2)
IF(1)
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Also for EHD1 (NM_006795)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human EHD1 was used as an immunogen.
Clone NameEPR4954 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens EH-domain containing 1 (EHD1), transcript variant 2
Alternative NameH-PAST; HPAST1; PAST; PAST1
Database LinkNP_006786
FunctionEHD1, or EH-domain containing 1, belongs to a highly conserved family of EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. EHD1 is thought to play a role in the endocytosis of IGF1 receptors (1).
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - EHD1 antibody [EPR4954]; All lanes : Anti-EHD1 antibody [EPR4954] at 1/1000 dilution.Lane 1 : HepG2 cell lysates.Lane 2 : Human testis lysates.Lane 3 : SW480 cell lysates.Lane 4 : SK-OV-3 cell lysates.Lane 5 : HeLa cell lysates.Lysates/proteins at 10 µg per lane.Predicted band size : 61 kDa.Observed band size : 60 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EHD1 antibody [EPR4954]; Immunohistochemical analysis of EHD1 in paraffin embedded Human kidney tissue, using TA307835 at a 1/100 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EHD1 antibody [EPR4954]; Immunohistochemical analysis of EHD1 in paraffin embedded Human tonsil tissue, using TA307835 at a 1/100 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - EHD1 antibody [EPR4954]; Immunofluorescent staining of EHD1 in HeLa cells, using TA307835 at a 1/100 dilution.

 

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