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Anti-EGR2 Antibody EPR4004
Also for EGR2 (NM_000399)
|A synthetic peptide corresponding to residues in human EGR2 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; FC: 1:100 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens early growth response 2 (EGR2), transcript variant 1|
|AT591; CMT1D; CMT4E; KROX20|
|EGR2 is a transcription factor, which the cDNA sequence predicts a protein of 406 amino acids, including three tandem zinc fingers of the Cys2-His2 class. The deduced amino acid sequences of human EGR2 and mouse Egr-1 are 92% identical in the zinc finger region but show no similarity elsewhere. Defects in EGR2 are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS) (1).|
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Western blot - EGR2 antibody [EPR4004]; All lanes : Anti-EGR2 antibody [EPR4004] at 1/1000 dilution.Lane 1 : LnCaP cell lysate.Lane 2 : HepG2 cell lysate .Lane 3 : MCF7 cell lysate.Lane 4 : SH SY5Y cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 53 kDa.
Flow Cytometry - Anti-EGR2 antibody [EPR4004]; Overlay histogram showing HepG2 cells stained with ab108399 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.