Home Antibody All anti-EGR-1 antibodies
Also for EGR-1 (NM_001964)
|This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 94-108 (eqpyehltaesfpdi) of Human EGR-1.|
|human, chimpanzee, mouse
||ELISA: 1:4,000 - 1:16,000, WB: 1:500 - 1:3,000, IHC: 2 ug/ml to 20 ug/ml
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|EGR-1 (also called Early Growth Response protein 1, Krox-24 protein, ZIF268, Nerve growth factor-induced protein A or NGFI-A, Transcription factor ETR103, and Zinc finger protein 225 or AT225) is a transcriptional regulator that recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3' (EGR-site). EGR-1 activates the transcription of target genes whose products are required for mitogenesis and differentiation. EGR-1 is a nuclear protein induced by growth factors. Expression has been identified in a variety of cancers.
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Western blot using Affinity Purified anti-EGR-1 antibody shows detection of a predominant band at ~58 kDa correspond-ing to EGR-1 present in mouse embryonic fibroblast whole cell lysate (arrowhead). Approximately 35 µg of lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Reaction occurred 2h at room temp-erature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Affinity Purified anti-EGR-1 antibody was used at a 10 ug/ml to detect nuclear and cytoplasmic signal with low background staining in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.