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Anti-EGLN2 Antibody EPR2746
Also for EGLN2 (NM_053046)
|A synthetic peptide corresponding to residues in human PHD1 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100; IP: 1:10
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt.
|Homo sapiens egl-9 family hypoxia-inducible factor 2 (EGLN2), transcript variant 1|
|EIT6; HIF-PH1; HIFPH1; HPH-1; HPH-3; PHD1|
Entrez Gene 112398 Human
Entrez Gene 112406 Mouse
Entrez Gene 308457 Rat
|Prolyl hydroxylase domain-containing protein 1 (PHD1, Hypoxia-inducible factor prolyl hydroxylase 1) is a 2-oxoglutarate and dioxygen-dependent enzyme that mediates the rapid destruction of hypoxia-inducible factor (HIF) alpha subunits (1) by catalyzing their post-translational formation. PHD1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It is also suggested to play a role in cell growth regulation (2). Loss of PHD1 has been shown to lower oxygen consumption in skeletal muscle by reprogramming glucose metabolism from oxidative to more anaerobic ATP production through activation of a Pparalpha pathway (3). Studies in a wide variety of cell lines revealed a predominant species at the predicted molecular mass (44 kDa) and an additional species running at a slightly increased mobility (40 kDa) (4).|
|Druggable Genome Pathways in cancerRenal cell carcinoma|
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Western blot - PHD1/prolyl hydroxylase antibody [EPR2746]; All lanes : Anti-PHD1/prolyl hydroxylase antibody at 1/1000 dilution.Lane 1 : A549 cell lysates.Lane 2 : HeLa cell lysates.Lane 3 : MCF-7 cell lysates.Lysates/proteins at 10 ug per lane.Predicted band size : 44 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PHD1/prolyl hydroxylase antibody [EPR2746]; TA307020, at a 1/100 dilution, staining PHD1/prolyl hydroxylase in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PHD1/prolyl hydroxylase antibody [EPR2746]; TA307020, at a 1/100 dilution, staining PHD1/prolyl hydroxylase in paraffin-embedded Human lung carcinoma tissue by Immunohistochemistry.