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Home Antibody All anti-EGLN2 antibodies

Anti-EGLN2 Antibody EPR2746

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307020
  • Rabbit monoclonal antibody against PHD 1/ 2/ 3(clone EPR2746 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403281) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(2)
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Also for EGLN2 (NM_053046)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human PHD1 was used as an immunogen.
Clone NameEPR2746 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100; IP: 1:10
Predicted MW Explanation 44 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens egl-9 family hypoxia-inducible factor 2 (EGLN2), transcript variant 1
Alternative NameEIT6; HIF-PH1; HIFPH1; HPH-1; HPH-3; PHD1
Database LinkNP_444274
Entrez Gene 112398 Human
Entrez Gene 112406 Mouse
Entrez Gene 308457 Rat
FunctionProlyl hydroxylase domain-containing protein 1 (PHD1, Hypoxia-inducible factor prolyl hydroxylase 1) is a 2-oxoglutarate and dioxygen-dependent enzyme that mediates the rapid destruction of hypoxia-inducible factor (HIF) alpha subunits (1) by catalyzing their post-translational formation. PHD1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It is also suggested to play a role in cell growth regulation (2). Loss of PHD1 has been shown to lower oxygen consumption in skeletal muscle by reprogramming glucose metabolism from oxidative to more anaerobic ATP production through activation of a Pparalpha pathway (3). Studies in a wide variety of cell lines revealed a predominant species at the predicted molecular mass (44 kDa) and an additional species running at a slightly increased mobility (40 kDa) (4).
Related PathwayDruggable Genome Pathways in cancerRenal cell carcinoma

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PHD1/prolyl hydroxylase antibody [EPR2746]; All lanes : Anti-PHD1/prolyl hydroxylase antibody at 1/1000 dilution.Lane 1 : A549 cell lysates.Lane 2 : HeLa cell lysates.Lane 3 : MCF-7 cell lysates.Lysates/proteins at 10 ug per lane.Predicted band size : 44 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PHD1/prolyl hydroxylase antibody [EPR2746]; TA307020, at a 1/100 dilution, staining PHD1/prolyl hydroxylase in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PHD1/prolyl hydroxylase antibody [EPR2746]; TA307020, at a 1/100 dilution, staining PHD1/prolyl hydroxylase in paraffin-embedded Human lung carcinoma tissue by Immunohistochemistry.

 

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